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pETNKI-his-SUMO-RNF168ring (1-189)
(Plasmid #63570)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63570 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Modifications to backbone
    Includes 6xHis-SUMO2 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNF168
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    570
  • Mutation
    Ring domain only (residues 1-189)
  • Entrez Gene
    RNF168 (a.k.a. RIDL, hRNF168)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • SUMO3 (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETNKI-his-SUMO-RNF168ring (1-189) was a gift from Titia Sixma (Addgene plasmid # 63570 ; http://n2t.net/addgene:63570 ; RRID:Addgene_63570)

  • For your References section:

    RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling. Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen W, Marteijn JA, Sixma TK. Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005. 10.1016/j.cell.2012.08.005 PubMed 22980979
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