pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R
(Plasmid
#63565)
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PurposeExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameH2A
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SpeciesH. sapiens (human)
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Insert Size (bp)394
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MutationLysines at 5, 9, 118, 119, 125, 127, and 129 are now Arginine
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Entrez GeneH2AC20 (a.k.a. H2A, H2A-GL101, H2A/q, H2AFQ, HIST2H2AC)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (MDYKDDDDKVPRIQ) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R was a gift from Titia Sixma (Addgene plasmid # 63565 ; http://n2t.net/addgene:63565 ; RRID:Addgene_63565) -
For your References section:
RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling. Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen W, Marteijn JA, Sixma TK. Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005. 10.1016/j.cell.2012.08.005 PubMed 22980979