pCDNA3.1-Flag-H2A K118-119R
(Plasmid #63564)

• Purpose: Expression of human H2A with K118R and K119R tagged with Flag
• Depositing Lab: Titia Sixma
• Publication: Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: H2A
• Species: H. sapiens (human)
• Insert Size (bp): 394
• Mutation: Lysine 118 and Lysine 119 are now Arginine
• Entrez Gene: H2AC20 (a.k.a. H2A, H2A-GL101, H2A/q, H2AFQ, HIST2H2AC)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (MDYKDDDDKVPRIQ) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry