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Addgene

pCDNA3.1-Flag-H2A K5-9R
(Plasmid #63562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    394
  • Mutation
    Lysine 5 and Lysine 9 are now Arginine
  • Entrez Gene
    H2AC20 (a.k.a. H2A, H2A-GL101, H2A/q, H2AFQ, HIST2H2AC)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (MDYKDDDDKVPRIQ) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1-Flag-H2A K5-9R was a gift from Titia Sixma (Addgene plasmid # 63562 ; http://n2t.net/addgene:63562 ; RRID:Addgene_63562)

  • For your References section:

    RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling. Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen W, Marteijn JA, Sixma TK. Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005. 10.1016/j.cell.2012.08.005 PubMed 22980979
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