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Addgene

pFusX1-33
(Plasmid #63251)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63251 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FusX1
  • Backbone size w/o insert (bp) 3013
  • Vector type
    TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NN NI NI
  • Alt name
    X1_GAA
  • Species
    Synthetic
  • Mutation
    none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer pCR8_F1
  • 3′ sequencing primer pCR8_R1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFusX1-33 was a gift from Stephen Ekker (Addgene plasmid # 63251 ; http://n2t.net/addgene:63251 ; RRID:Addgene_63251)

  • For your References section:

    FusX: A rapid one-step TALE assembly system for genome science. Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP, Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker SC. Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172 10.1089/hum.2015.172 PubMed 26854857
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