pAV10.K5
(Plasmid
#63210)
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Purpose(Empty Backbone) pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCACS
- Backbone size (bp) 4200
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Modifications to backboneBsaI and BsmBI sites removed from backbone, RFP cloned into the MCS, flanked by BsaI sites for cloning TUs using yGG assmebly
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Growth instructionsGrows as red/pink colonies
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV10.K5 was a gift from Jef Boeke (Addgene plasmid # 63210 ; http://n2t.net/addgene:63210 ; RRID:Addgene_63210) -
For your References section:
Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ, Martin JA, Caravelli K, Stracquadanio G, Boeke JD. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23. 10.1021/sb500372z PubMed 25756291
