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Addgene

pAV10.K5.loxP
(Plasmid #63204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCACS
  • Backbone size (bp) 5200
  • Modifications to backbone
    BsaI and BsmBI sites removed from backbone, RFP cloned into the MCS, flanked by BsaI sites for cloning TUs using yGG assmebly, TU flanked with loxP sites.
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Grows as red/pink colonies
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV10.K5.loxP was a gift from Jef Boeke (Addgene plasmid # 63204 ; http://n2t.net/addgene:63204 ; RRID:Addgene_63204)

  • For your References section:

    Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ, Martin JA, Caravelli K, Stracquadanio G, Boeke JD. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23. 10.1021/sb500372z PubMed 25756291
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