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PurposeExpresses CcaS, Ho1, and PcyA constitutively
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYCDuet-1
- Total vector size (bp) 6282
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCcaS
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SpeciesSynechocystis sp. PCC 6803
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Insert Size (bp)2262
Gene/Insert 2
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Gene/Insert nameHo1
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SpeciesSynechocystis sp. PCC 6803
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Insert Size (bp)723
Gene/Insert 3
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Gene/Insert namePcyA
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SpeciesSynechocystis sp. PCC 6803
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Insert Size (bp)747
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSR43.6r was a gift from Jeffrey Tabor (Addgene plasmid # 63197 ; http://n2t.net/addgene:63197 ; RRID:Addgene_63197) -
For your References section:
Refactoring and optimization of light-switchable escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. 10.1021/sb500273n PubMed 25250630