pAV124
(Plasmid #63190)

• Purpose: (Empty Backbone) pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
• Depositing Lab: Jef Boeke
• Publication: Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.

Backbone

• Vector backbone: pRS424
• Backbone size (bp): 5500
• Modifications to backbone: BsaI and BsmBI sites removed from backbone, RFP cloned into the MCS, flanked by BsaI sites for cloning TUs using yGG assmebly
• Vector type: Bacterial Expression, Yeast Expression, Synthetic Biology
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: Grows as red/pink colonies
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pAV124

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry