Or46a-Gal4
(Plasmid #63179)

• Purpose: Expresses Gal4 under control of the Drosophila melanogaster Or46a odorant receptor promoter
• Depositing Lab: Leslie Vosshall
• Publication: Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

Backbone

• Vector backbone: pCasperAUG-VP22-Gal4
• Backbone manufacturer: Leslie Vosshall
• Backbone size w/o insert (bp): 10000
• Total vector size (bp): 11888
• Modifications to backbone: This vector contains a fusion of the first ~30 amino acids of Drosophila melanogaster alcohol dehydrogenase gene, followed by the Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, followed by the S. cerevisiae (budding yeast) Gal4 gene. This translational fusion generates a single protein: AUG-Adh-VP22-Gal4
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Or46a promoter
• Species: D. melanogaster (fly)
• Insert Size (bp): 1888

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pCasper-F 5'-gggttttattaacttacat-3'
• 3′ sequencing primer: GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Or46a-VP22-Gal4 consists of the 1.888 Kb Drosophila melanogaster Or46a promoter subcloned into the EcoRI site of the pCaSpeR-AUG-VP22-Gal4 vector. This VP22-Gal4 is a fusion of the HSV-1 structural protein VP22 (Elliott and O’Hare, 1997; PMID: 9008163) to yeast Gal4. The activity of this fusion protein is indistinguishable from that of unmodified Gal4 and did not show the intercellular protein transfer properties of unmodified VP22 (Elliott and O’Hare, 1997; PMID: 9008163).