pMarsL274G
(Plasmid #63177)

• Purpose: This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.
• Depositing Lab: David Tirrell
• Publication: Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 10.1021/jacs.5b08980. Epub 2016 Mar 25.

Backbone

• Vector backbone: pcDNA3.1+
• Total vector size (bp): 8086
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MarsL274G
• Alt name: Mars
• Alt name: methionine-tRNA synthetase (L274G)
• Species: M. musculus (mouse)
• Insert Size (bp): 2753
• Mutation: L274G
• GenBank ID: NP_001003913
• Entrez Gene: Mars1 (a.k.a. Mars, Metrs, Mtrns)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: xho1 (not destroyed)
• 5′ sequencing primer: GCTCTCTGGCTAACTAGAGAACCCACTGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Developed by Alborz Mahdavi.