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PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepProTet.E333
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Backbone manufacturerClontech
- Total vector size (bp) 3936
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCcaR
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SpeciesSynechocystis sp. 6803
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Insert Size (bp)705
Gene/Insert 2
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Gene/Insert namesfGFP
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Alt nameSuperfolder GFP
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SpeciesSynthetic
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Insert Size (bp)720
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSR58.6 was a gift from Jeffrey Tabor (Addgene plasmid # 63176 ; http://n2t.net/addgene:63176 ; RRID:Addgene_63176) -
For your References section:
Refactoring and optimization of light-switchable escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. 10.1021/sb500273n PubMed 25250630