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PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB4A3
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Backbone manufacturerRegistry of Standard Biological Parts
- Total vector size (bp) 5363
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameOmpR
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Insert Size (bp)720
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Entrez GeneompR (a.k.a. b3405, ECK3392, cry, kmt, ompB)
Gene/Insert 2
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Gene/Insert namesfGFP
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Alt nameSuperfolder GFP
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SpeciesSynthetic
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Insert Size (bp)720
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSR59.4 was a gift from Jeffrey Tabor (Addgene plasmid # 63175 ; http://n2t.net/addgene:63175 ; RRID:Addgene_63175) -
For your References section:
Refactoring and optimization of light-switchable escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. 10.1021/sb500273n PubMed 25250630