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Addgene

BLBP-GFP
(Plasmid #63174)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63174 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG-GFP
  • Backbone manufacturer
    https://www.addgene.org/11150/
  • Backbone size w/o insert (bp) 1700
  • Total vector size (bp) 5411
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BLBP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Entrez Gene
    Fabp7 (a.k.a. B-FABP, BFABP, Blbp, MRG)
  • Promoter 1700bp upstream of BLBP gene

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CAATGTCGACAGCACAGCAGAAAGGGAAAA
  • 3′ sequencing primer GGTGGGCGCGCCAGGCAGGAACTGGAGGAACTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BLBP-GFP was a gift from Bart De Strooper (Addgene plasmid # 63174 ; http://n2t.net/addgene:63174 ; RRID:Addgene_63174)
  • For your References section:

    APLP2 regulates neuronal stem cell differentiation during cortical development. Shariati SA, Lau P, Hassan BA, Muller U, Dotti CG, De Strooper B, Gartner A. J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77. doi: 10.1242/jcs.122440. Epub 2013 Jan 23. 10.1242/jcs.122440 PubMed 23345401