pDestTol2CG2-U6:gRNA Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pDestTol2CG2-U6:gRNA with the following enzymes: SpeI-HF and XhoI.
We expect and see bands at 3.4, 2.7, and 2.2 kb.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid
pDestTol2CG2-U6:gRNA with the following enzymes: EcoRI-HF
and Sac II.
Expected results:
EcoRI-HF/Sac II 4659 + 3601 bp
We observed the expected bands.