pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
(Plasmid #63139)

• Purpose: Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)
• Depositing Lab: Titia Sixma
• Publication: Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub 2006 May 18.

Backbone

• Vector backbone: pGEX-6p-2
• Backbone manufacturer: GE Healthcare Life Sciences
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Ring1b
• Species: M. musculus (mouse)
• Insert Size (bp): 486
• Mutation: Residues 1-159
• Entrez Gene: Ring1 (a.k.a. Ri, Ring1A)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Gene/Insert 2

• Gene/Insert name: Bmi1
• Species: M. musculus (mouse)
• Insert Size (bp): 330
• Mutation: Residues 1-109
• Entrez Gene: Bmi1 (a.k.a. Bmi-1, Pcgf4)
• Promoter: tac

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry