pET3CTc3A(1-135)
(Plasmid
#63138)
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PurposeExpression of human Tc3(1–135) bipartite domain tagged with 6xHis
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET3C
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTc3A
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Alt nameTransposable element Tc3 transposase
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SpeciesC. elegans (nematode)
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Insert Size (bp)426
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Mutation1-135 aa bipartite domain
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET3CTc3A(1-135) was a gift from Titia Sixma (Addgene plasmid # 63138 ; http://n2t.net/addgene:63138 ; RRID:Addgene_63138) -
For your References section:
Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA. Watkins S, van Pouderoyen G, Sixma TK. Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004. 10.1093/nar/gkh770 PubMed 15304566