pGEX6p1-GST-Rnf31
(Plasmid #63134)

• Purpose: Expression of human RNF31/HOIP codon optimized for E. coli and insect cells
• Depositing Lab: Titia Sixma
• Publication: Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi: 10.1038/emboj.2012.217. Epub 2012 Aug 3.

Backbone

• Vector backbone: pGEX-6p-1
• Backbone manufacturer: GE Healthcare
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rnf31
• Alt name: HOIP
• Alt name: ZIBRA
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3216
• Mutation: Synthetic based on human protein sequence
• Entrez Gene: RNF31 (a.k.a. HOIP, IMD115, Paul, ZIBRA)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry