pTNS3
(Plasmid #63127)

• Purpose: (Empty Backbone) Tn7 transposase expression
• Depositing Lab: Herbert Schweizer
• Publication: Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1064-75. Epub 2007 Dec 21.

Backbone

• Vector backbone: pTNS2
• Modifications to backbone: strong promoter P1 was added to drive transposase expression
• Vector type: Bacterial Expression
• Promoter: P1 and Ptac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Growth instructions: requires pir-positive E. coli for replication
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.