c7-132/202_HLL
(Plasmid
#63123)
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PurposeMm-cpn double cysteine mutant for crosslinking
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a
- Total vector size (bp) 7038
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMm-cpn c7_132/202_HLL
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Insert Size (bp)1630
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MutationC140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S202C
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Entrez GeneNEWENTRY
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Tag
/ Fusion Protein
- 6x HIS-tag (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
c7-132/202_HLL was a gift from Tanja Kortemme (Addgene plasmid # 63123 ; http://n2t.net/addgene:63123 ; RRID:Addgene_63123) -
For your References section:
Reprogramming an ATP-driven protein machine into a light-gated nanocage. Hoersch D, Roh SH, Chiu W, Kortemme T. Nat Nanotechnol. 2013 Dec;8(12):928-32. doi: 10.1038/nnano.2013.242. Epub 2013 Nov 24. 10.1038/nnano.2013.242 PubMed 24270642