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Purpose(Empty Backbone) mini-Tn7 lacZ transcriptional fusion vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC18T-miniTn7T-Gm
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Modifications to backbonepromoterless lacZ was inserted within miniTn7 element
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsalso ampicillin resistant
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC18-mini-Tn7T-Gm-lacZ was a gift from Herbert Schweizer (Addgene plasmid # 63120 ; http://n2t.net/addgene:63120 ; RRID:Addgene_63120) -
For your References section:
A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923
