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Addgene

Frt-V5-hspB5
(Plasmid #63106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Modifications to backbone
    FRT is N-term on HygroB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hspb5
  • Species
    H. sapiens (human)
  • Entrez Gene
    CRYAB (a.k.a. CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Frt-V5-hspB5 was a gift from Harm Kampinga (Addgene plasmid # 63106 ; http://n2t.net/addgene:63106 ; RRID:Addgene_63106)

  • For your References section:

    HSPB7 is the most potent polyQ aggregation suppressor within the HSPB family of molecular chaperones. Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH. Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. Epub 2010 Sep 15. 10.1093/hmg/ddq398 PubMed 20843828
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