Frt-V5-hspB4
(Plasmid
#63105)
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PurposeExpression of HSPB4 (small HSP) tagged with V5 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Modifications to backboneFRT is N-term on HygroB
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehspb4
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SpeciesH. sapiens (human)
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Entrez GeneCRYAA (a.k.a. CRYA1, CTRCT9, HSPB4)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Frt-V5-hspB4 was a gift from Harm Kampinga (Addgene plasmid # 63105 ; http://n2t.net/addgene:63105 ; RRID:Addgene_63105) -
For your References section:
HSPB7 is the most potent polyQ aggregation suppressor within the HSPB family of molecular chaperones. Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH. Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. Epub 2010 Sep 15. 10.1093/hmg/ddq398 PubMed 20843828
