pAAV CCTG 1200x
(Plasmid #63088)

• Purpose: AdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2
• Depositing Lab: Nicolas Charlet-Berguerand
• Publication: Charlet-Berguerand plasmids (unpublished)

Backbone

• Vector backbone: unknown
• Total vector size (bp): 8924
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CCUG 1200x
• Species: Synthetic
• Insert Size (bp): 4598
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pAAV CMV CCUG 1200 annotated genbank file
• Addgene Notes:
  • Addgene Diagnostic Test
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.

This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.