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Addgene

pKG136
(Plasmid #63075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIC242
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-W
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    267
  • Mutation
    E. coli codon optimized, hardened against Dharmacon siRNA pool
  • Entrez Gene
    CENPW (a.k.a. C6orf173, CENP-W, CUG2)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • TEV-S (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer oCB21
  • 3′ sequencing primer oCB22
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG136 was a gift from Iain Cheeseman (Addgene plasmid # 63075 ; http://n2t.net/addgene:63075 ; RRID:Addgene_63075)
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