pIC80
(Plasmid
#63064)
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PurposeMammalian expression of EGFP-TEV-S-tag DSN1 for protein localization and affinity purification (LAP)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEYFP-C2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDSN1, IMAGE clone 6063417
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Alt nameQ9H410
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SpeciesH. sapiens (human)
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Insert Size (bp)1071
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Entrez GeneDSN1 (a.k.a. C20orf172, KNL3, MIS13, dJ469A13.2, hKNL-3)
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Tags
/ Fusion Proteins
- EYFP (N terminal on backbone)
- TEV-S (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer oCB21
- 3′ sequencing primer oCB22 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIC80 was a gift from Iain Cheeseman (Addgene plasmid # 63064 ; http://n2t.net/addgene:63064 ; RRID:Addgene_63064)