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Or83a-Gal4
(Plasmid #63050)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63050 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCasperAUG-Gal4-X
  • Backbone manufacturer
    Leslie Vosshall Lab
  • Backbone size w/o insert (bp) 12000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila melanogaster Or83a promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1628
  • Tag / Fusion Protein
    • Drosophila ADH (aa's 1-18) fused to Yeast Gal4 (aa's 1-881) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCasper-F 5'-gggttttattaacttacat-3'
  • 3′ sequencing primer GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Or83a-Gal4 was a gift from Leslie Vosshall (Addgene plasmid # 63050 ; http://n2t.net/addgene:63050 ; RRID:Addgene_63050)

  • For your References section:

    Chemotaxis behavior mediated by single larval olfactory neurons in Drosophila. Fishilevich E, Domingos AI, Asahina K, Naef F, Vosshall LB, Louis M. Curr Biol. 2005 Dec 6;15(23):2086-96. 10.1016/j.cub.2005.11.016 PubMed 16332533
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