Or42b-Gal4
(Plasmid
#63033)
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PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or42b, using the promoter sequences of the gene cloned 5' of Gal4
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63033 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCasperAUG-Gal4-X
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Backbone manufacturerLeslie Vosshall Lab
- Backbone size w/o insert (bp) 12000
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrosophila melanogaster Or42b promoter
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SpeciesD. melanogaster (fly)
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Insert Size (bp)8039
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Tag
/ Fusion Protein
- Drosophila ADH (aa's 1-18) fused to Yeast Gal4 (aa's 1-881) (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCasper-F 5'-gggttttattaacttacat-3'
- 3′ sequencing primer GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Or42b-Gal4 was a gift from Leslie Vosshall (Addgene plasmid # 63033 ; http://n2t.net/addgene:63033 ; RRID:Addgene_63033) -
For your References section:
Genetic and functional subdivision of the Drosophila antennal lobe. Fishilevich E, Vosshall LB. Curr Biol. 2005 Sep 6;15(17):1548-53. 10.1016/j.cub.2005.07.066 PubMed 16139209