Or42a-Gal4
(Plasmid #63032)

• Purpose: To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or42a, using the promoter sequences of the gene cloned 5' of Gal4
• Depositing Lab: Leslie Vosshall
• Publication: Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

Backbone

• Vector backbone: pCasperAUG-Gal4-X
• Backbone manufacturer: Leslie Vosshall Lab
• Backbone size w/o insert (bp): 12000
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Drosophila melanogaster Or42a promoter
• Species: D. melanogaster (fly)
• Insert Size (bp): 4184
• Tag / Fusion Protein:
  • Drosophila ADH (aa's 1-18) fused to Yeast Gal4 (aa's 1-881) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pCasper-F 5'-gggttttattaacttacat-3'
• 3′ sequencing primer: GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry