pBABEPuro-rnSOX9
(Plasmid
#63009)
-
PurposeExpresses rat SOX9
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5169
-
Vector typeRetroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSOX9
-
SpeciesR. norvegicus (rat)
-
GenBank IDKP732536
-
Entrez GeneSox9 (a.k.a. SRY)
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB I (blunt) (unknown if destroyed)
- 3′ cloning site SnaB I (blunt) (unknown if destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE-3' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBABEPuro-rnSOX9 was a gift from Michael Ruppert (Addgene plasmid # 63009 ; http://n2t.net/addgene:63009 ; RRID:Addgene_63009) -
For your References section:
SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM. J Cell Sci. 2015 Jan 27. pii: jcs.162164. 10.1242/jcs.162164 PubMed 25632159