H6-epsin 1 (144-575)
(Plasmid #63008)

• Purpose: expresses the adaptor protein epsin that lacks its ENTH-domain in bacteria
• Depositing Lab: Ernst Ungewickell
• Publication: Kalthoff et al J Biol Chem. 2002 Mar 8;277(10):8209-16. Epub 2001 Dec 26.

Backbone

• Vector backbone: pQE-32
• Backbone manufacturer: Qiagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Epsin 1 without ENTH domain
• Species: R. norvegicus (rat)
• GenBank ID: NM_057136.1
• Entrez Gene: Epn1
• Tag / Fusion Protein:
  • His tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBRforEco (5'-AATAGGCGTATCACGAGGC-3')
• 3′ sequencing primer: T0term-R (5'-GCGGCAACCGAGCGTTC-3)'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Pietro de Camilli Chen et al.:Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis JOURNAL Nature 394 (6695), 793-797 (1998)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry