H6-AP180
(Plasmid
#63006)
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Purposeexpresses the adaptor protein AP180 in bacteria
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQE-31
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAP180
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SpeciesR. norvegicus (rat)
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GenBank IDXP_006243556.1
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Entrez GeneSnap91 (a.k.a. Ap180)
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Tag
/ Fusion Protein
- His-tag (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H6-AP180 was a gift from Ernst Ungewickell (Addgene plasmid # 63006 ; http://n2t.net/addgene:63006 ; RRID:Addgene_63006) -
For your References section:
Inositol hexakisphosphate binds to clathrin assembly protein 3 (AP-3/AP180) and inhibits clathrin cage assembly in vitro. Norris FA, Ungewickell E, Majerus PW. J Biol Chem. 1995 Jan 6;270(1):214-7. PubMed 7814377