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Addgene

pPyCAG-Nanog(T100A)-IP
(Plasmid #62989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPyCAG-Nanog-IP
  • Backbone manufacturer
    Shinya Yamanaka
  • Backbone size w/o insert (bp) 6378
  • Total vector size (bp) 7359
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse Nanog T100A Mutant
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    918
  • Mutation
    Changed Threonine 100 (101AA) to Alanine
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
  • Promoter CAG

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pPyCAG-Nanog-IP (Addgene # 13838)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPyCAG-Nanog(T100A)-IP was a gift from Shinya Yamanaka (Addgene plasmid # 62989 ; http://n2t.net/addgene:62989 ; RRID:Addgene_62989)
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