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Addgene

pSpCas9(BB)-2A-Puro (PX459) V2.0 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 62988 with enzyme EcoRI-HF.

We expected the following band sizes for this digest: 0.7 and 8.5bp. We see the expected pattern. Note that the smaller band is very faint on this gel image.