pMOS010: pLX304-CheRiff-GFP
(Plasmid #62986)

• Purpose: Lentivirus production of CheRiff channelrhodopsin actuator
• Depositing Lab: Adam Cohen
• Publication: Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 2014 Jun 22.

Backbone

• Vector backbone: pLX304
• Backbone manufacturer: David Root
• Backbone size w/o insert (bp): 9377
• Total vector size (bp): 9511
• Vector type: Mammalian Expression, Lentiviral ; Gateway Destination vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CheRiff-eGFP
• Alt name: ChR64
• Species: Synthetic
• Insert Size (bp): 1734
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry