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pcDNA3.1 myc-mβTrCP WD1-7 (241-569)
(Plasmid #62977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BTRC
  • Species
    M. musculus (mouse)
  • Mutation
    codes for residues 241-569
  • GenBank ID
    NM_009771.3
  • Entrez Gene
    Btrc (a.k.a. Beta-Trcp1, E3RS-IkappaB, E3RSIkappaB, FWD1, Fbw1a, HOS, SCF b-TRCP, Slimb, b-TrCP, beta-TrCP)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 myc-mβTrCP WD1-7 (241-569) was a gift from Michael Ruppert (Addgene plasmid # 62977 ; http://n2t.net/addgene:62977 ; RRID:Addgene_62977)

  • For your References section:

    SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM. J Cell Sci. 2015 Jan 27. pii: jcs.162164. 10.1242/jcs.162164 PubMed 25632159
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