pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
(Plasmid
#62956)
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PurposeSinglet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepC-(loxP2-attB2-SA(2))
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Backbone manufacturerBenjamin White
- Backbone size w/o insert (bp) 8608
- Total vector size (bp) 11805
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2A-Gal4-Hsp70
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Insert Size (bp)3191
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70) was a gift from Benjamin White (Addgene plasmid # 62956 ; http://n2t.net/addgene:62956 ; RRID:Addgene_62956) -
For your References section:
Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes. Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH. Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.059 PubMed 25732830
Map uploaded by the depositor.
