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Addgene

pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70
(Plasmid #62953)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-KS-attB1-2-PT-SA-SD-2
  • Backbone manufacturer
    Hugo Bellen
  • Backbone size w/o insert (bp) 3394
  • Total vector size (bp) 8748

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3XGal80-FLAG/HA/EGFP-Hsp70
  • Insert Size (bp)
    5348

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70 was a gift from Benjamin White (Addgene plasmid # 62953 ; http://n2t.net/addgene:62953 ; RRID:Addgene_62953)

  • For your References section:

    Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes. Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH. Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.059 PubMed 25732830
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