pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70
(Plasmid #62948)

• Purpose: Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1
• Depositing Lab: Benjamin White
• Publication: Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.

Backbone

• Vector backbone: pBS-KS-attB1-2-PT-SA-SD-1
• Backbone manufacturer: Hugo Bellen
• Backbone size w/o insert (bp): 3394
• Total vector size (bp): 5119

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T2A-LexA::QFAD-Hsp70
• Insert Size (bp): 1711

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated by the laboratory of Christopher Potter and is based on the LexA::QFAD construct (http://www.addgene.org/46123/) published at: http://dx.doi.org/10.1038/nmeth.3250

Other reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.