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pJEP221-AAV-RSV-GFP-pA
(Plasmid #62919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 3166
  • Total vector size (bp) 3952
  • Modifications to backbone
    Vector backbone has 75 bps 3’UTR that contains an SV40 based poly-adenylation signal
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green Fluorescent protein (GFP)
  • Insert Size (bp)
    786
  • Promoter Rous Sarcoma Virus(RSV)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer actGAATTCGCCGCCACCAT GGT GAG CAA GGG CGA GGA G
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJEP221-AAV-RSV-GFP-pA was a gift from Jonathan Ploski (Addgene plasmid # 62919 ; http://n2t.net/addgene:62919 ; RRID:Addgene_62919)

  • For your References section:

    The production of viral vectors designed to express large and difficult to express transgenes within neurons. Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE. Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7. 10.1186/s13041-015-0100-7 PubMed 25887710
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