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Addgene

pJW6.01
(Plasmid #62882)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62882 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pID2.02
  • Backbone manufacturer
    Seydoux lab, Addgene plasmid # 26955
  • Backbone size w/o insert (bp) 8260
  • Vector type
    Worm Expression
  • Selectable markers
    unc-119

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP::MEG-3
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    6609
  • Promoter meg-3
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

InFusion cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW6.01 was a gift from Geraldine Seydoux (Addgene plasmid # 62882 ; http://n2t.net/addgene:62882 ; RRID:Addgene_62882)
  • For your References section:

    Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G. Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591. 10.7554/eLife.04591 PubMed 25535836