pJW3.42
(Plasmid
#62866)
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PurposeYeast two-hybrid bait vector expressing LexA-PPTR-1 fusion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLexA-N Gateway compatible destination vector
- Backbone size w/o insert (bp) 5650
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Vector typeYeast Expression ; two-hybrid bait vector
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePPTR-1/pLexA-N bait
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SpeciesC. elegans (nematode)
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Insert Size (bp)1631
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Tag
/ Fusion Protein
- LexA (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer LexA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJW3.42 was a gift from Geraldine Seydoux (Addgene plasmid # 62866 ; http://n2t.net/addgene:62866 ; RRID:Addgene_62866) -
For your References section:
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G. Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591. 10.7554/eLife.04591 PubMed 25535836
Map uploaded by the depositor.
