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Addgene

pJW3.31
(Plasmid #62865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pY3H Gateway compatible destination vector
  • Backbone size w/o insert (bp) 8128
  • Vector type
    Yeast Expression ; two-hybrid bridge vector
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAA-1/Y3H
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1828
  • Promoter TEF

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TEF_seq (5'-ggtcttcaatttctcaagtttc-3')
  • 3′ sequencing primer CYC1 (5'-GCGTGAATGTAAGCGTGAC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW3.31 was a gift from Geraldine Seydoux (Addgene plasmid # 62865 ; http://n2t.net/addgene:62865 ; RRID:Addgene_62865)

  • For your References section:

    Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G. Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591. 10.7554/eLife.04591 PubMed 25535836
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