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Addgene

pCMV CytoBAS
(Plasmid #62860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6251
  • Modifications to backbone
    Synthetic DNA construct encoding BAS-1 was cloned into pECFP-C1, replacing ECFP, to generate NucleoBAS. CytoBAS was created by KpnI digestion of NucleoBAS, which removed the NLS.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CytoBAS
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV CytoBAS was a gift from Stan van de Graaf (Addgene plasmid # 62860 ; http://n2t.net/addgene:62860 ; RRID:Addgene_62860)

  • For your References section:

    Monitoring bile acid transport in single living cells using a genetically encoded Forster resonance energy transfer sensor. van der Velden LM, Golynskiy MV, Bijsmans IT, van Mil SW, Klomp LW, Merkx M, van de Graaf SF. Hepatology. 2013 Feb;57(2):740-52. doi: 10.1002/hep.26012. Epub 2013 Jan 8. 10.1002/hep.26012 PubMed 22899095
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