CMV-LUC2CP/ARE
(Plasmid #62857)

• Purpose: Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
• Depositing Lab: Gideon Dreyfuss
• Publication: Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. Epub 2010 Feb 1.

Backbone

• Vector backbone: pGL4.16
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4758
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Luciferase
• Alt name: firefly luciferase
• Species: firefly
• Insert Size (bp): 2157
• Mutation: destabilizing sequences (DS) added to the C terminus of the protein (CL1 and PEST) and five consecutive AUUUA elements added to the 3′ UTR (RNA DS ARE) for rapid mRNA turnover
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Promega
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry