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Addgene

pRS-mTagRFP-TUBA1B
(Plasmid #62850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS424
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5616
  • Vector type
    yeast-E.coli shuttle vector
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTagRFP-TUBA1B
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2180
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning method: DNA assembly by transformation in yeast.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS-mTagRFP-TUBA1B was a gift from Gaudenz Danuser (Addgene plasmid # 62850 ; http://n2t.net/addgene:62850 ; RRID:Addgene_62850)

  • For your References section:

    Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration. Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, Danuser G. Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 10.1016/j.cels.2016.08.007. 10.1016/j.cels.2016.08.007 PubMed 27667364
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