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Addgene

pHL2117
(Plasmid #62842)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown-ColE1 ori
  • Backbone manufacturer
    Lim Lab
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    M8::ryhB leader
  • Promoter pConShortNoHind

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    gfp
  • Promoter pLtetO-1
  • Tag / Fusion Protein
    • m9::sodB leader

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL2117 was a gift from Han Lim (Addgene plasmid # 62842 ; http://n2t.net/addgene:62842 ; RRID:Addgene_62842)

  • For your References section:

    Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Sagawa S, Shin JE, Hussein R, Lim HN. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. 10.1093/nar/gkv777 PubMed 26261213
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