pHL2075
(Plasmid
#62838)
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PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown-ColE1 ori
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Backbone manufacturerLim Lab
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameglmZ leader
- Promoter pConShortNoHind
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer AspTerm (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameT710 hfq leader
- Promoter pLlacO-1
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer T1ter-rev (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert namegfp
- Promoter pLtetO-1
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Tag
/ Fusion Protein
- glmS (N terminal on insert)
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL2075 was a gift from Han Lim (Addgene plasmid # 62838 ; http://n2t.net/addgene:62838 ; RRID:Addgene_62838) -
For your References section:
Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Sagawa S, Shin JE, Hussein R, Lim HN. Nucleic Acids Res. 2015 Aug 10. pii: gkv777. 10.1093/nar/gkv777 PubMed 26261213
Map uploaded by the depositor.
