pHL1969
(Plasmid #62832)

• Purpose: Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1
• Depositing Lab: Han Lim
• Publication: Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv777.

Backbone

• Vector backbone: unknown-ColE1 ori
• Backbone manufacturer: Lim Lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: M8::ryhB leader
• Promoter: pConNoHind

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: AspTerm

Gene/Insert 2

• Gene/Insert name: T710 hfq leader
• Promoter: pLlacO-1

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: T1ter-rev

Gene/Insert 3

• Gene/Insert name: gfp
• Promoter: pLtetO-1
• Tag / Fusion Protein:
  • m9::sodB leader (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: GFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry