pCMK
(Plasmid #62818)

• Purpose: Bacterial constitutive S. pyogenes Cas9 expression plasmid
• Depositing Lab: Sébastien Rodrigue
• Publication: Bacterial constitutive gRNA expression plasmid (unpublished)

Backbone

• Vector backbone: pCMK
• Total vector size (bp): 6735
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas9
• Species: Synthetic; Streptococcus pyogenes
• Insert Size (bp): 4141

Resource Information

• Supplemental Documents:
  • Annotated pCMK
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry